Angela Holuba
Barnard College
(2008)
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During May-July 2008, Angela Holuba will take part
in an investigation of the phylogeny of the
scorpion family Buthidae. Holuba, supervised by
doctoral student Lauren Esposito and Associate
Curator Lorenzo Prendini, will work on the
molecular component of the investigation. During
this time she will learn to extract, isolate,
amplify, sequence, and edit DNA. Holuba will
additionally learn techniques for phylogenetic
analysis.
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Sylvia Johnson
Barnard College
(Collegiate Science and Technology Entry Program, 2008)
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From May to August 2008, Sylvia Johnson will work
on a project investigating the phylogeny and
evolutionary relationships of the scorpion family
Diplocentridae. Over the course of the summer,
Johnson will learn to extract DNA, amplify and
sequence genes from samples collected throughout
southern North America, the Caribbean, and the
Middle East. She will also learn to edit and
phylogenetically analyze the data she has
collected. Johnson's research will be supervised by
doctoral student Lauren Esposito and Associate
Curator Lorenzo Prendini.
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Gena Esposito
University of Texas at Austin
(NSF RevSys Grant, 2007)
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In June and July, 2007, Gena Esposito took part in
an investigation of the phylogeny of the scorpion
family Buthidae using molecular data. Esposito used
samples collected from all over the world to
sequence molecular data from six different loci.
She learned how to extract, isolate, amplify,
sequence, and edit DNA. She also learned to clone
DNA fragments using bacterial vectors.
Esposito’s research was supervised by
Associate Curator Lorenzo Prendini and her sister,
doctoral student Lauren Esposito.
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Sarah Shoenbrun
Brown University
(NSF Research Experiences for Undergraduates Internship, 2007)
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During June–August 2007, Sarah Schoenbrun
studied the only North American scorpion genus of
medical importance, Centruroides, and
analyzed molecular data from previously
unidentified specimens to clarify their
phylogenetic placement within the genus. Working
under the supervision of doctoral student Lauren
Esposito and Associate Curator Lorenzo Prendini,
Schoenbrun further investigated the relationship
between venom genes of Centruroides and
other medically important scorpions by using
cloning techniques to sequence a scorpion
α-toxin multi-gene family, ultimately
examining these evolutionary relationships within
the known framework of scorpion phylogeny.
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Steve Webb
Muhlenberg College
(NSF Research Experiences for Undergraduates Internship, 2005)
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During June–August 2005, Steve Webb studied
the phylogenetic placement and monophyly of the
buthoid scorpion family Microcharmidae, using both
morphological and molecular data. Webb’s
research project was supervised by AMNH
Postdoctoral Fellows Erich Volschenk and Camilo
Mattoni, and Associate Curator Prendini. During his
internship, Webb learned to isolate, amplify,
sequence, and edit scorpion DNA, to examine and
score morphological character data, and to analyse
both sources of data phylogenetically in a total
evidence framework.
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| Kanvaly B. Bamba
Yale University (NSF Research Experiences for Undergraduates Internship, 2004) Michelle McCoy North Carolina University (NSF Research Experiences for Undergraduates Internship, 2004) |
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During June–August 2004, Kanvaly Bamba and Michelle
McCoy worked together, under the supervision of Lorenzo
Prendini, Scientific Assistant Randy Mercurio, and
Laboratory Assistants Tarang Sharma and Rebecca Budinoff,
to produce the first phylogeny for the Gondwana scorpion
family Liochelidae, based on a simultaneous analysis six
gene loci and morphology. Both students learned to
photograph scorpions under visible and UV light, to
measure scorpions and record trichobothrial counts, to
isolate, amplify, sequence, and edit DNA, and to analyse
morphological and molecular data phylogenetically. They
subsequently traveled to southern California for two
weeks to collect scorpions with Randy Mercurio and
California Academy of Sciences Field Associate, Warren
Savary, a trip supported by the Richard Lounsbery Foundation.
Samara Maaliki
City University of New York
(Joint NSF Research Experiences for Undergraduates Internship and Undergraduate Mentoring in Evolutionary Biology Internship, 2003)
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During June–August 2003, Samara Maaliki
studied the morphology of the monotypic central
Asian scorpion family Pseudochactidae and
investigated its phylogenetic position in
Scorpiones with morphological and molecular data,
under the supervision of Lorenzo Prendini,
Scientific Assistant Randy Mercurio, and former
Laboratory Assistants Kelly DeMeo and Tarang
Sharma. Maaliki learned to measure scorpions and
photograph them under visible and UV light, to
isolate, amplify, sequence, and edit DNA, and to
analyse morphological and molecular data
phylogenetically.
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Lauren A. Esposito
University of Texas, El Paso
(NSF Research Experiences for Undergraduates Internship, 2002)
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During June–August 2002, Lauren Esposito
reinvestigated the phylogeny of the medically
important African buthid scorpion genus
Parabuthus from a combined molecular and
morphological perspective, under the supervision of
Lorenzo Prendini, and assisted by Kelly DeMeo and
Anne Keller. Esposito learned to identify
Parabuthus species using morphological
characters by sorting the AMNH holdings of
Parabuthus. She also learned to isolate,
amplify, sequence, and edit DNA, and to analyse
morphological and molecular data phylogenetically.
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